Single wells which could not be enumerated because of confluence phenomena were assessed by using the highest numbers of cytokine-producing cells which could be regularly counted in other wells in the same assay as an approximated estimate

Single wells which could not be enumerated because of confluence phenomena were assessed by using the highest numbers of cytokine-producing cells which could be regularly counted in other wells in the same assay as an approximated estimate. Statistical analysis For

2002;99:2866C2871

2002;99:2866C2871. described (11). The PCR primers and conditions are available upon request. siRNA and Transient Transfections HCC827 and BT-474 cells were transfected with 50nM silencer select validated siRNA or negative control (Ambion) with HiPerFect Transfection Reagent (Qiagen) according to manufacturers