Dissected tissue was grown on 0

Dissected tissue was grown on 0.4?m Biopore filters (Millipore, UK) in 2.5?ml of serum-free culture medium (DMEM/Hams F12 1:1) containing 1 x ITS (insulin, transferrin and sodium selenite) (Sigma, UK), 0.025?mg/ml gentamicin (Sigma, UK), 0.06?mg/ml benzylpenicillin sodium, 0.1?mg/ml streptomycin sulphate