Lamp1-GFP accumulation at vacuoles was not inhibited by treatment of cells with brefeldin A, which disrupts the Golgi network, suggesting that Lamp1-GFP recruitment is not due to trafficking from your Golgi but instead occurs by lysosome fusion (Figure 3, F and G, Supplemental Figure S5C, and Supplemental Video S5)

Lamp1-GFP accumulation at vacuoles was not inhibited by treatment of cells with brefeldin A, which disrupts the Golgi network, suggesting that Lamp1-GFP recruitment is not due to trafficking from your Golgi but instead occurs by lysosome fusion (Figure 3, F