Comparison of stable condition surface-associated matriptase while dependant on biotinylation with S-NHS-SS-biotin towards the accumulated biotin-RQRR-CMK labeled matriptase more than a 30 min period or much longer demonstrates only a small fraction of surface-associated matriptase on Caco-2 cells can bind biotin-RQRR-CMK (shape 2A, compare street 1 to lanes 4C6)

Comparison of stable condition surface-associated matriptase while dependant on biotinylation with S-NHS-SS-biotin towards the accumulated biotin-RQRR-CMK labeled matriptase more than a 30 min period or much longer demonstrates only a small fraction of surface-associated matriptase on Caco-2 cells can bind